Research on the Test of Hepatic Function with Chromazurol S.　-Report Ⅱ. Changes of liver functions in carbon tetrachloride dosed goats.-

1. A slight hepatic disturbance occurrred following an intramuscular injection of CCL4, 0.5 ml. Per kg, in goats. The change of chromazurol S (CS) concentrations in the blood of goats loaded with 10 mg/dl CS per kg was the same as that in dogs, and reflected the degree of liver damage due to CCL4 administration. However, disappearance of the dye from blood seemed to be rapid. 2. Using both CS and fructose tolerance tests, impairment of liver functions was demonstrated from 6 hours after the injection of CCL4, and recovery of the test results was seen after 5 days. 3. Protein metabolism as judged from various serum colloid reactions disclosed a dissociation among the tests results during 12 hours to 5 days after CCL4 administration, and the recovery was delayed. The sensitivity of these tests may be of the following order: CS retention test, >fructose tolerance test, >A/G ratio, >Sawada's mercuric chloride reaction,=cadmium reaction,=Takata-Ara reaction, >BSP, >Kunkel's zinc sulphate test,=thymol turbidity test, etc. 4. The BSP test showed a slight increase of retention during 12 to 24 hours in some animals and no changes in the other. Chromazurol S test is therefore superior to BSP test. 5. Urinary urobilinogen was variable and unreliable. 6. If thest test were analyzed on the basis of liver cell morphology, CS test was found to be correlated well with the degree of fatty metamorphosis. However, CS retention returned to normal levels earier than the recovery of histological alterations.