1. Tolerance of Paramecium Caudatumto hydrogen and hydroxyl ions I. E. PH change of environmental culture fluid was examined by means of microscopic observation. 2. Formulas are presented to express approximetely the tolerance (per cent probability of cessation response of ciliary motion) of paramecia in terms of time of exposure and concentration of hydrogen and hydroxyl ions. 3. The avobe formulas were able to be constructed by applying Chung's general formulas concerning stress and response.