Monoclonal Antibody May Recognize Highly Evolutionary Conserved Molecule - An Immunocytochemical Study -

A monoclonal antibody, UB-13 which may react highly evolutionary conserved molecule was established. The antibody was made immunizing mice with homogenized skate brain and then cloning both with rat and skate thymocytes. The reactivity of the antibody was analyzed immunocytochemically using the indirect immunocytochemical method. The UB-13 appeared to be reactive with thymocytes of all species of vertebrates examined, such as fish, frog, turtle, chicken and rat. Cells of the nervous system, some populatons of lymphoid cells are also positive with UB-13 immunostaining. These findings strongly suggest that UB-13 is a unipue monoclonal antibody which recognizes evolutionary conserved molecule.

Monoclonal antibody

Evolutionary conserved antigen

Thymocytes

Brain

Immunocytochemistry