Studies on the Interaction between ФBA1 of Bacillus aneurinolyticus and Its Killing－Sensitive Host

In order to investigate the mechanism of killing of ФBA1 against several strains of Bacillus aneurinolyticus, the possibility of ФBA1to propagate on Bacillus aneurinolyticus KA10, which was one of the killing-sensitive hosts of the phage, was examined. Electron microscopy exhibited no propagation of mature particles of ФBA1in KA10 cells examined. Endogenous high molecular weight bacteriocin lysogenized in KA10 neither conferred immunity against ФBA1nor was induced in the course of killing by ФBA1.Phenomana similar to Weigle-reactivation in Escherichia coli of ФBA1 in UV-irradiated productive host (KA23) could be observed, whereas no enhancement of killing activity was observed in the killingsensitive host. Those results taken together, there was no indication of the expression of ФBA1 genome in the cell of killing sensitive host KA10. Thus the possibility of propagation of ФBA1 in B. aneurinolyticus KA10 was ruled out in the present experimental condition.

Bacillus aneurinolyticus

bacteriocin

bacteriophage

immunity

reactivation