Factors Influencing Patency of Femoropopliteal Arterial Reconstructions

Tritiated-thymidine uptake rate at various points within the S phase was measured in exponentially growing hamster kidney carcinoma cells in vitro (HKC 400) by a new retrograde synchrony method. Although the method is analogous to the labeled mitoses technique, it utilizes the date of radioactivity per labeled mitosis obtained by cytophotometric measurement of silver grains in autoradiogram. The uptake rate showed biphasic pattern: the first peak which was the highest was in the mid-S phase and the second was just before the end of S. There was a marked depression in the late S phase and it was as low as 5% of the first peak. This marked depression suggested the possibility that the S phase is dividedinto at least 2 subphases with regards to the utilization of the exogenous thymidine.

early and late occlusion

femoropopliteal bypass

autogenous saphenous vein

dacron graft