1). Rf values of the poisonous conponents of globefish (Spheroides rubripes) livers and ovaries were determined chromatographically with the aid of mouse assay and they were compared with the Rf value of crystalline tetrodotoxin. 2). Irrespective of individual toxic potency and the organ, the Rf value was founded to the same as that of crystalline tetrodotoxin. 3). These results storongly indicated that the liver poison is identical to the ovary poison, or that these poisons are very closely related in chemical structure.