Diphtheria toxoid contained a main component which migrated toward anode with a velocity somewhat larger than that of serum albumin. By the one-dimensional crossing electrophoresis, ''crossing diagrams'' of diphtheria antiserum and those of toxin and of toxoid were obtained. In the crossing diagrams of antiserum appeared each two peaks in the region of β-and or γ-globulin. In the crossing diagrams of toxoid appeared one peak in the region of β-globulin and two very flat peaks in the region of γ-globulin. The antigens corresponding to these flat peaks appeared to be contained in the toxin.