The bulletin of the Yamaguchi Medical School

Continued by:Medical Science & Innovation
EISSN:2758-5441

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The bulletin of the Yamaguchi Medical School Volume 33 Issue 1
published_at 1986-12

Relationship between C-MYC Expression and the Cell Cycle in HL-60 Cells -Flow Cytometric Analysis-

Relationship between C-MYC Expression and the Cell Cycle in HL-60 Cells -Flow Cytometric Analysis-
Murakami Tomoyuki
Ogino Tetsuro
Takahashi Manabu
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A020033000102.pdf
Descriptions
By flow cytometric measurement of cellular DNA, c-myc protein and cell volume, we attempted to assess the relationship between c-myc gene expression and the cell cycle in exponentially growing human promyrlocytic leukemia cell line (HL-60). The protein product of c-myc gene was stained by an indirect immunofluorescence method using a specific antiserum to human c-myc protein. Bivariate distributions of cellular DNA, the c-myc protein contents and cell volume demonstrated the direct evidence that the c-myc protein increases with the progression of the cell cycle and then is halved by cell division.
Creator Keywords
Oncogene
c-myc protein
Cell cycle
HL-60
Flow cytometry