Relationship between C－MYC Expression and the Cell Cycle in HL－6０ Cells －Flow Cytometric Analysis－

Relationship between C－MYC Expression and the Cell Cycle in HL－6０ Cells －Flow Cytometric Analysis－

Creators Sasaki Kohsuke

Creators Murakami Tomoyuki

Creators Ogino Tetsuro

Creators Takahashi Manabu

By flow cytometric measurement of cellular DNA, c-myc protein and cell volume, we attempted to assess the relationship between c-myc gene expression and the cell cycle in exponentially growing human promyrlocytic leukemia cell line (HL-60). The protein product of c-myc gene was stained by an indirect immunofluorescence method using a specific antiserum to human c-myc protein. Bivariate distributions of cellular DNA, the c-myc protein contents and cell volume demonstrated the direct evidence that the c-myc protein increases with the progression of the cell cycle and then is halved by cell division.

[ISSN]0513-1812

[NCID]AA00594273