The bulletin of the Yamaguchi Medical School

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The bulletin of the Yamaguchi Medical School Volume 31 Issue 1
published_at 1984-12

Flow Cytometric Study on Tumor Antigen TA－4 in Uterine Cervical Squamous Cells

Flow Cytometric Study on Tumor Antigen TA－4 in Uterine Cervical Squamous Cells
Sasaki Kohsuke
Nagai Michihiro
Kato Hiroshi
Torigoe Tadashi
Nagamine Yoshiaki
Takahashi Manabu
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About This Article
Descriptions
Flow cytometric analysis was made of a tumor-antigen, TA-4, in cells from squamous cell carsinoma of the utrine cervix. The cells were stained by the indirect immunofluorescent antibody technique using a rabbit antibody specific to TA-4 and fluorescein isothiocyanate conjugated anti-rabbit IgG antibody. FCM histograms of the carcinoma cells showed a broad but conspicuous pesk with intense fluorescence pattern not seen in normal squamous epithelium. Would be possible to distinguish malignant from non-malignant cells of the uterine cervix it by the FCM analysis. Possible application of the technique to cytological screening of cervical cancer is discussed.
Creator Keywords
Flow cytometry
Tumor antigen
Squamous cell carcinoma
Uterine cervical cancer
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