Experimental Studies of Inducing Agents of Guinea Pig Liver Changed by Immunological Technique

Guinea Pig liver proteins, which had been prepared by isoelectric precipitation after centrifugation at low or high speed, were used as the immunizing antigen in rabbits. Antisera fomed against these antigens were used for precipitin reaction with the low or high speed protein antigen.These antigens, antisera and antigen-antibody complexes were examined for their induction effects on Triturus gastrula. Antiserum against liver ”low speed protein'' showed very low frequency and poor differentination in the induction, and the same inducing tendency was also observed in the case of antiserum against liver ”high speed protein''. When the antigens were coupled with antibodies, mesodermal inducing ability appeared only in the antigen-antibody complex with one rabbit antiserum but in others the inducing ability was depressed and the frequency of appearance of induced structure was very low.