1. Tip junction potential of the microelectrode widely used in electro-physiological research, and intracellular potential of Nitella flexilis were studied by using an appropriate circuit (shown in figure 1). 2. Some electrodes had a high tip potential and were not suitable. Others with relatively low potentials might be used in the experiments with adequate correction.3. A linear relationship was demonstrated between the intracellular potential of Nitella and the logarithm of K+ concentration in the extrancellular solution.