The bulletin of the Yamaguchi Medical School
Continued by：Medical Science & Innovation
EISSN：2758-5441
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The bulletin of the Yamaguchi Medical School Volume 47 Issue 3-4
published_at 2000-12
TNF-α and IL-8 Release by Sinusoidai Endothelial Cells during Hypoxia-reoxygenation in Rats with Obstructive Jaundice
TNF-α and IL-8 Release by Sinusoidai Endothelial Cells during Hypoxia-reoxygenation in Rats with Obstructive Jaundice
Hayashi Daisuke
About This Article
Descriptions
The release of of cytokines by sinusoidal endothelial cells (SECs) during hypoxia reoxygenation was studied in vitro in male Wistar rats with obstructive jaundice to investigate the kinetics of tumor necrosis factor-α (TNF-α) and interleukin-8 (IL-8) release by SECs during hypoxia-reoxygenation. The rats were divided into two groups according to the procedure perfomed. An obstructive jaundice group (Group OJ) underwent bile duct ligation and a control group (Group C) underwent a sham-operation. SECs were isolated by collagenase digestion and centrifugal elutriation. After exposing the cells to hypoxia in 95% oxygen. The levels of TNF-α and IL-8 were significantry decreased in group OJ compared with those in Group C, indicationing that obstructive jaundice induced functional damage to SECs with decreased cytokine production during hypoxia-reoxygenation.
Creator Keywords
ischemia-reperfusion injury
sinusoidal endothelial cells
cytokine
obstructive jaundice
hypoxia-reoxygenationhi
Other Article
PP. 69 - 79