TNF-α and IL-8 Release by Sinusoidai Endothelial Cells during Hypoxia-reoxygenation in Rats with Obstructive Jaundice
The bulletin of the Yamaguchi Medical School Volume 47 Issue 3-4
Page 81-87
published_at 2000-12
Title
TNF-α and IL-8 Release by Sinusoidai Endothelial Cells during Hypoxia-reoxygenation in Rats with Obstructive Jaundice
Creators
Hayashi Daisuke
Source Identifiers
Creator Keywords
ischemia-reperfusion injury
sinusoidal endothelial cells
cytokine
obstructive jaundice
hypoxia-reoxygenationhi
The release of of cytokines by sinusoidal endothelial cells (SECs) during hypoxia reoxygenation was studied in vitro in male Wistar rats with obstructive jaundice to investigate the kinetics of tumor necrosis factor-α (TNF-α) and interleukin-8 (IL-8) release by SECs during hypoxia-reoxygenation. The rats were divided into two groups according to the procedure perfomed. An obstructive jaundice group (Group OJ) underwent bile duct ligation and a control group (Group C) underwent a sham-operation. SECs were isolated by collagenase digestion and centrifugal elutriation. After exposing the cells to hypoxia in 95% oxygen. The levels of TNF-α and IL-8 were significantry decreased in group OJ compared with those in Group C, indicationing that obstructive jaundice induced functional damage to SECs with decreased cytokine production during hypoxia-reoxygenation.
Languages
eng
Resource Type
departmental bulletin paper
Publishers
Yamaguchi University Graduate School of Medicine
Date Issued
2000-12
File Version
Version of Record
Access Rights
open access
Relations
[ISSN]0513-1812
[NCID]AA00594272
Schools
医学部