The bulletin of the Yamaguchi Medical School

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EISSN:2758-5441

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The bulletin of the Yamaguchi Medical School Volume 1 Issue 3
published_at 1953-12-01

A SIMPLE PROCEDURE FOR THE ESTIMATION OF SERUM CHOLINESTERASE BY MEANS OF COMPARATOR WITH PHENOL RED AS INDICATOR

A SIMPLE PROCEDURE FOR THE ESTIMATION OF SERUM CHOLINESTERASE BY MEANS OF COMPARATOR WITH PHENOL RED AS INDICATOR
Shibata Susumu
Takahashi Hiroshi
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A020001000307.pdf
Descriptions
 A routine procedure for the estimation of serum cholinesterase was described. Its pronciple is placed, like those of the methods having been developed by Michel and Alcalde, on the measurement to pH drop in the buffered substrate mixed with serum. Its teehnic is simple and efficient, because it employs a comparator to determine pH instead of glass electrode pH-meter. Special caution was taken with regard to the composition of buffered substrate in order that the fall of pH might indicate hydrolysis. This method has been in use for three years in our laboratory with satisfactory results for the diagnosis of hepatic disturbances. Normal serum cholinesterase activity fell within the pH between 0.8 and 1.1.