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A SIMPLE PROCEDURE FOR THE ESTIMATION OF SERUM CHOLINESTERASE BY MEANS OF COMPARATOR WITH PHENOL RED AS INDICATOR

The bulletin of the Yamaguchi Medical School Volume 1 Issue 3 Page 188-197
published_at 1953-12-01
A020001000307.pdf
[fulltext] 705 KB
Title
A SIMPLE PROCEDURE FOR THE ESTIMATION OF SERUM CHOLINESTERASE BY MEANS OF COMPARATOR WITH PHENOL RED AS INDICATOR
Creators Shibata Susumu
Creators Takahashi Hiroshi
Source Identifiers
 A routine procedure for the estimation of serum cholinesterase was described. Its pronciple is placed, like those of the methods having been developed by Michel and Alcalde, on the measurement to pH drop in the buffered substrate mixed with serum. Its teehnic is simple and efficient, because it employs a comparator to determine pH instead of glass electrode pH-meter. Special caution was taken with regard to the composition of buffered substrate in order that the fall of pH might indicate hydrolysis. This method has been in use for three years in our laboratory with satisfactory results for the diagnosis of hepatic disturbances. Normal serum cholinesterase activity fell within the pH between 0.8 and 1.1.
Subjects
医学 ( Other)
Languages eng
Resource Type departmental bulletin paper
Publishers Yamaguchi University Graduate School of Medicine
Date Issued 1953-12-01
File Version Version of Record
Access Rights open access
Relations
[ISSN]0513-1812
[NCID]AA00594272
Schools 医学部