Specific Reaction of a Protein of Jack Bean with Sera of Various Animals

Jack bean contained a protein, which reacted specifically with serum proteins. This specific protein was named provisionally Protein J. Specific precipitation of serum proteins with Protein J was confirmed by simply mixing them in test tube or by the technique of agar gel diffusion. By the two-dimensional crossing paper electrophoresis, crossing diagrams of the extract of jack bean against sera on the hand, and those of sera against the extract of jack bean on the other hand, were obtained. The foemer diagrams showed a peak in the region of Protein J, indicating that the reactant was Protein J