Improved Technique of O-Aminobiphenyl Method for the Determination of Glucose in Serum

The o-aminobiphenyl colorimetric method for the determination of glucose in serum which was developed by SHIBATA was improved by the use of color reagent recommended by SHIRAKATA, which is amixture of 880 ml of 0.5 g/dl o-aminobiphenyl solution (dissolved in glacial acetic acid) with 120 ml saturated aqueous solution of boric acid containing 360 mg of sodium fluoride. In the improved method 50λof blood serums is added to 5.0ml of the color regent, heated in a boiling water bath for 30 minutes, and the greenish color thus developed in the solution is subjected to photoelectric colorimeter with a red filter (660mμ). This enables us an accurate glucose determination of serum of plasma in the presence of fluoride as well as in its absence.