Supplentary Studies of the Saponin-Induced Colpnization of the Bone Marrow Elements in Rabbits

1. A large dose (6 mg) of saponin (E.. MERCK) was administered in a single, intravenous injection into adult albino rabbits, which had been intravenously injected with 200μc of inorganic Pの32 in a single dose 24 hours previously. 2. In less then 72 hours after saponin, there occurred a striking increase in the amount of radioactivity in the spleen and to a lesser extent also in the liver, whereas the amount of radioactivity in the bone marrow was invariably reduced to a large extent, as compared with the values for the control group without saponin injection. 3. The histological examination of various organs revealed striking accumulation of immature blood cells in the spleen and liver, with a simultaneous depletion of these cells in the bone marrow. 4. The above-mentioned pattern of accumulation of immature blood cells in foreign organs after saponin injection was the same as that observed for the transfused marrow cells.