Fluorescent Microscopic Studies on Various Exudate Cells in the Inflammatory Focus. Ⅱ.observations on the Immature Cells of Bone Marrow and Lymph Node

The fiuorescent findings of the immature blood cells in the bone marrow and lymph node of human, mouse and rat were described. Each cell type emits characteristic fluorescence. The fluorescence of the cells of lymphocytic series is most intense, that of the monocytic series is very feeble, and that of the myeloid series is of the middle grade. The cytoplasm of the immature cells generally emits green fluorescence, whereas that of mature cells gives red fluorescence. It should be noticed, however, that the cytoplasm of the mature lymphocyte emits green fluorescence except for the azur granules, which sometimes show red fluorescence. The plasma cell is easily discriminated from the other series by the presence of non-fluorescent large Golgi complex. As regards the differences in the fluorescent color of the cell, the action of the Van der Waals' force between the size of the protein molecule and the dye seems to play an important role in producing such differences.The dead cells show homogenous red fluorescence, so that the discrimination of the living and that the discrimination of the living and dead cells can easily be made by the fluorescent method. Besides, labelling of the cell can easily be made without injurying of the living cells by the use of fluorescent dyes.