Immunohistochemical Demonstration of Nerves Containing Protein Gene Product 9.5 (PGP 9.5) and Neuropeptides in the Mouse Kidney

The immunoreactivity for proｔein gene product (PGP) 9.5, a neuronal marker, was examined in the mouse kidney. Immunoreactivities for neuropeptide Y (NPY), calcitonin gene-related peptide (CCRP),substance P (SP) and vasoactive intestinal polypaptide (VIP) were also studied.Nerve fibers with PGP 9.5 immunoreactivity were widely recognized in all segments of the renal vascular system,i.e.,in the interlobar,arcuate,and interlobar arteries and veins,in the afferent and efferent arterioles,and in the vasa recta of the outer stripe of the outer zone of the medulla.In the renal tebules, there ware also a few varicose terminals showing PGP 9.5 immunoreactivity.NPY-immunoreactive fibers occurred widly in the renal vascular system forming a plexus.Moderate number of CGRP-immunoreactive fibers and a few SP-immunoreactive fivers,which seemd to be co-localized partly,were distributed mainly in the interlobar and adcuate arteries.No VIP-positive nerves were recognizable.Each positive type of nerve fibers shouwed an unique distribution and seemed to act differently in the various branches of the renal vascular system.

Protein gene product 9.5-Neuropeptide Y-Calcitonin gene-related peptide-Substance P-Kidney innervation