Healing of Cooley Double Velour Arterial Graft in Man

The effect of hydrocortisone on the blastogenesis of human peripheral blood lymphocytes in PHA culture was studied by morphological and cytochemical techniques. Hydrocortisone markedly inhibited morphological transformation of lymphocytes in PHA culture for up to 72 hours. The extent of the inhibition of blastogenesis depends on the concentrations of the hydrocortisone and on the added time intervals after PHA stimulation. The inhibitory effect of hydrocortisone practically disappeared by removing the hydrocortisone from the culture medium before PHA stimulation. A distinct, but transient, decrease in fast green histone content in small lymphocytes, about 75% of the control value, was obtained at 30 minutes after PHA stimulation. The increase in Feulgen-DNA content of the lymphocytes was clearly observed in PHA culture for 72 hours. However, such histone changes and thereafter increase of DNA value were not observed when hydrocortisone was added with PHA simultaneously or 30 minutes before PHA. Present results suggest that hydrocortisone may inhibit histone changes of small lymphocytes in early stages after PHA, and suppresses DNA synthesis of the PHA-stimulated lymphocytes.

cooley double velour graft

pseudoneointima

outer and inner capsules

scanning electron micrograph

complete healing