Further Evidences for the Cholonergic and Adrenergic Mechanisms in the Isolated Hypogastric Nerve-Seminal Vesicle Preparation of the Guinea-Pig

Effects of autonomic on the responses produced by hupogastric nerve and transmural stimulation of the hypogastric nerve-seminal vesicle preparation og the guinea-pig were examined. Hexamethonium and nicotine blocked the responeses to hyprogastric stimulation leaving the contractions due to transmural stimulation. TTX and xylocaine blocked both responses. Spontaneous contraction which was observed in 30% of the preparations was not aboloshed by TTX, hexamethonium, guanethidine and atropine. Acetrlcholine, metacholine and noradrenaline elicited or potentiated the spontanous activity. Adrenaline, noradrenaline, phynylephrine and nialamide enhanced the reponses produced by the stimuration of hypogastic and transmural stimulation while guanethidine and phenoxybenzamine reduced the responses. Actylcholine, metacholine, carbachol and cholinesterase inhibitors increased the responses. Atropine and bevonium inhibited the responses. Required puse number in the transmural stimulation for proucing the contraction was decreased in nialaide, metacholine, carbachol, physostigmine and neostigmine, and increased in phenoxybenzamine, guanethidine and atropine. The possibility that adrenergic and cholinergic mechanisms operate independently of each other in the seminal vesicle preparation was discussed. Greateful acknowledgement is made to Prof. C.L. Prosser, Department of Physiology and Biophysics, University of Illonois, for his kind guidance and careful review of the manuscript in this investigation.