Identification of Abnormal Hemoglobins IV. Polyacrylamide Gel Electrophoresis of the Globin Chains

Polyacrylamide gel electrophoresis of the globin chains using two buffer systems, tris-glycine (pH9.8) and β-alanne acetate (pH4.6) in concentrated urea, was studied by column and slab methods. Concentration of urea was reduced from 8 M to 6.4 M for convenience without change in resolving power. In order to save time, total acrylamide concentration of 4.9g/dl was preferred to 7.3g/dl. The pH 9.8 buffer was quite suitable for the separation of the α,β and σ chains, while the pH 4.6 buffer was particularly useful for the separation of the γ from the βchain and of the N-acetyland γ chain from the unmodified one…