Analysis of Intra-cellar Influx of Adriamycin after Electrical Impulse Chemotherapy

To investigate the mechanism of electrical impluse chemotherapy, we administered such treatment using adriamycin (ADR) to rats inoculated AH109-A ascites hepatorr.a. The carcinostatic effect and intracellular influx of ADR were investigate by means of flowcytometry (FCM) and fluorescent microscopy. When rats bearing the AH109-A subcutaneous solid tumor were treated with ADR followed by spike wave loading, the T/C ratio (the tumor volume of the treatment group comnpared with that of the control group) on post-treatment day 7 was0.37±0.07, which　was　significantly lowerascompared with the values of 0.49±0.05 taken in the group given ADR alone (p<0.01). In rats inoculated AH109-A hepatoma into : abdiminal cavity given in vivo electrical impluse chemotherapy with ADR, intracellular concentration of drug measured by FCM was about 3 times higher than observed with the drug given alone. When electrical impluse chemotherapy was applied in vitro to AH109-A cells exposed to ADR, 1.25mg/ml, fluorescence microscory reveaiec that 50±0 cells were positive for the drug, significantly greater than the 21±9 cells observed v-. Ith the administration of ADR alone (p<0.01). It is suggested that spike wave electricla impulse chemotherapy induce eiectroporation of the cell membrane and permit the intracellular influx of a large amount of carcin: static agent which exerts a strong carcinostatic effect.

電撃化学療法

電気的穿孔現象

アドリアマイシン