Chemical Identification of Abnormal Hemoglobin A new cord blood hemoglobin Hb F Kogobuki γ6 (A3) Glu → Gly

We recently found two varient hemoglobin in a survet of 1,970 cord bloods collected in Yamaguchi prefecture. One was a fast moving varient like Hb J and the other a slow moving hemoglobin similar to Hb S. The former proved to be a varient of αchain and the latter that of γ chain by urea polyacrylamide gel electrophoresis of the globin. The γchain varient was purified by DE-52 column chromatography. It emerged between Hb A_2 and Hb A (A_0) and comparised approximately 10% of total hemoglobin or 17% of Hb F. The γ chain was prepared by CM-52 column chromatography and studied by amino acid analysis,…