Glycolipids are cellular membrane components that may play an important role in the development, differentiation, proliferation, and carcinogenesis of cells. Qualitative analysis of glycolipids and sulfatide in 22 primary colorectal cancer and 6 adjacent normal mucosa was performed using Thin Layer Chromatography (TLC) and TLC immuno-staining. Quantitative analysis was performed by a densitometric scanning method. Ceramide monohexoside (CMH) was significantly decreased and ceramide dihexoside (CDH) was significantly increased in cancerous tissues compared with normal ones (P<0.001). MD ratio [CMH / (CMH + CDH)] in 14 manifested lymph node metastasis was higher than in 7 which the above was absent (P< 0.05). And also in Dukes stage, MD ratio in 9 Dukes C was higher than in 7 Dukes A(P<0.05). Two spots of sulfatide (CSE) were observed with TLC immuno-staining method and we designated each spot as CSE-A and CSE-B. CSE-A was significantly decreased and CSE-B was significantly increased in cancerous tissues compared with normal tissues (P<0.05). CSE ratio [CSE-B / (CSE-A+ CSE-B)] in 15 lymph node metastasis cases was higher than that of 7 cases lacking metastasis(P<0.05). And also in Dukes stage, CSE ratio in 9 Dukes C and 6 Dukes D were higher than in 7 Dukes A(P<0.02, P<0.05). Change of glycolipids on the cellular membrane, especially change of sulfatide, were correlated with malignancy potential of colorectal cancer.