Specium Method for Electorn　Microscopy PartII. On the Supporting Membranes of Samples 3. A New Method with Highly Efficient Techniques for Membrane Making

A　highly efficient method　of membrane making for the specimen preparaiton in transmission electron microscopy was contrived. The method includes　the following procedures of techniques: 1） Spreading of a collodion film on the water surface 2) Regular placing of copper grids on the collodion film 3) Overlapping of a round filter paper　on the above　4)Sinking of the filter paper into the water of vessel　5) Salvaging of the above and transferring into a Petri dish 6) Drying,carbon-coating and preservation of the filter paper. This　method provides much more advantageous effects comparison with the conventional method. A large number of grids can be handled at a time of preparation and the　following treatments, such as mount of samples, shadowing and negative staining , are very convenient. Therefore, this would be emphasized as a new method in the membrance making.