Hypoxic cell radiosensitizer RK28 cysteine conjugate : its isolation and collection

We have reported that RK28 cysteine conjugate(metabolite e)might be a novel hypoxic cell radiosensitizer as its concentration in tumor was higher than in serum on the rabbit VX2 tumor system. In this study, we report the results of its isolation and collection from rabbit urine after RK28 intravenous injection. RK28 and major metabolites were analyzed using high performance liquid chromatography(HPLC) with tetrabutylammonium phosphate solution(TBAP). RK28 cysteine conjugate was isolated using a LH20 column, a G10 column, and an ODS column without TBAP. A total amount of 211μg was collected but not enough for further studies.

低酸素細胞

放射線増感剤

RK28

代謝物

本文データは山口大学医学会の許諾に基づきCiNiiから複製したものである