Hypoxic cell radiosensitizer RK28 cysteine conjugate : its isolation and collection
山口医学 Volume 49 Issue 5
Page 491-496
published_at 2000-10-31
Title
放射線増感剤RK28システイン抱合体 : その分離と分取について
Hypoxic cell radiosensitizer RK28 cysteine conjugate : its isolation and collection
Creators
Ebe Kazuyu
Creators
Fujisawa Keiko
Creators
Nagano Hiromi
Creators
Karino Yuichi
Creators
Tsukahara Masato
Creators
Miyata Yoshiyuki
Creators
Tsujitani Michihiko
Source Identifiers
Creator Keywords
低酸素細胞
放射線増感剤
RK28
代謝物
We have reported that RK28 cysteine conjugate(metabolite e)might be a novel hypoxic cell radiosensitizer as its concentration in tumor was higher than in serum on the rabbit VX2 tumor system. In this study, we report the results of its isolation and collection from rabbit urine after RK28 intravenous injection. RK28 and major metabolites were analyzed using high performance liquid chromatography(HPLC) with tetrabutylammonium phosphate solution(TBAP). RK28 cysteine conjugate was isolated using a LH20 column, a G10 column, and an ODS column without TBAP. A total amount of 211μg was collected but not enough for further studies.
Languages
jpn
Resource Type
journal article
Publishers
山口大学医学会
Date Issued
2000-10-31
Rights
本文データは山口大学医学会の許諾に基づきCiNiiから複製したものである()
File Version
Version of Record
Access Rights
open access
Relations
[ISSN]0513-1731
[NCID]AN00243156
[isVersionOf]
[NAID]http://ci.nii.ac.jp/naid/110002774008/
Schools
医学部