Involvement of Intracellular Signal Transducing System in Nitrous Oxide-Induced Analgesia and Tolerance

The intracellular signal transducing (1ST) system has recently been suggested to play an important role in pain transmission and modulation. N_2O has been known to have analgesic action. We are unaware of any report which has examined the effect of N_2O on the 1ST system. The present study was designed to determine 3^H -clonidine, 3^H-GTP, and 3H-forskolin bindings in the rat CNS, using in vitro autoradiography, in N_2O analgesia. Wistar rats were exposed to either 75% N_2 (control) or 75% N_2O. In the N_2O group, rats were initially exposed for 2 hrs (first exposure group) and re-exposed for 2 hrs (second exposure group) after a 1hr interval. N_2O produced analgesia: the percent maximal possible effect (%MPE

笑気麻酔

耐性

in vitro autoradiogradiography

α_2受容体

アデニル酸シクラーゼ