We have carried out the studies on the effect of sodium hydrosulfite and sodium hydroxide concentrations in the reducing reagent upon the bule color development, and besides, on the separation and purification by batch operation with Dowex 50 W-4 (200-400mesh). The results were as follows. 1. The composition of sodium hydrosulfite and sodium hydroxide concentrations in the reducing reagent was variously changed and the influence of these concentrations on the intensity of color and the stability of color was investigated. The absorbances of paraquat solution at 5 minutes after the development of color showed the same values in the cases of all reducing reagents…