A Study was undertaken regarding the determination, identification, purification, and separation of paraquat. The results were as follows. 1. Separation and purfication of paraquat : Ionexchange resin culumn chromatography with Dewex 50 W-4 resin was used. After applying 10 ml of 0.002% paraquat : solution, it was washed with a small quantity of water and then the stepwise elution was made with each 60 ml of 1, 2, 3, 4, 5, 6, and 7N hydrochloric acid at a rate of 3ml/min. The elute was fractionated into 5ml portions and the absorbance of fraction was measured at 260 mμ. Paraquat was eluted in fractions 40 (4N hydrochloric acid) to 55 (5N hydrochloric acid)…