A Study on the Method for the Identification of Bacterial Thiaminase

To the identification of thiaminase-producing bacilli, the detection and the typing of thiaminase of cultures are indispensable. The author has adopted a new method fot the estimation of the thiaminase activity in the culture fluid of thiaminase-producing basilli. The method depends essentially on the detection of thiazole which is one of the enzymatic products from thiamine added to the culture fluid. Aothough the method is not quantitative, it is easy to perform and is convenient especially when many bacterial cultures must be studied. The method comprises two steps