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Permalink : https://petit.lib.yamaguchi-u.ac.jp/24383
山口医学 Volume 63 Issue 3
published_at 2014-08-01
スライスパッチクランプ法を用いた一次運動野ニューロンとシナプス機能の解析 (テクニカルノート)
スライスパッチクランプ法を用いた一次運動野ニューロンとシナプス機能の解析 (テクニカルノート)
About This Article
Descriptions
パッチクランプ法は細胞レベルで脳機能を解明するための有用なツールである.パッチクランプ実験を成功させるために,本稿では1)良質な脳サンプル標本を得るための注意点,2)顕微鏡下における脳スライス標本の観察の方法,3)細胞へのアプローチとパッチ膜の形成の手順を解説する.さらに細胞特性やシナプス機能解析の実験例を挙げて実験手法を紹介する.
Creator Keywords
シナプス可塑性
パッチクランプ
AMPA受容体
GABAA受容体
Other Article
PP. 207 - 211