DNA ploidy using paraffin-embedded tissues in urological cancer : 3. bladder cancer

The DNA analysis by flow cytometry (FCM) was conducted using paraffin blocks of resected tissues from 73 patients with bladder cancer. They had received trans-urethral ressection (TUR-Bt) or partial cystectmoy as an initial treatment in our Department of during 10 years from January 1975 to December 1984. Of 73 patients, 18 underwent total cystectomy after conservative treatmentand 55 received transurethral resection. The results obtained were follows. (1) DNA ploidy analysis was in 94% (17/18) of cases with total cystectomy and in 36% (20/55) of cases with transurethral resection, with significantly lower frequency (p＜0.005). (2) The statistical significant relationship was not observed between histological grade and DNA ploidy. (3) The histological stage and DNA ploidy showed that DNA aneuploidy in 30% (6/20) in T1 and below T1, and in 59% (10/17) in T2 and above T2 with significantly higher frequency (p＜0.05). (4) The relationship between the prognosis and DNA ploidy showed that five year survival rate was 71% in DNA diploidy cases and 22% in DNA anuuploidy cases, with tendency toward poorer prognosis in those with DNA aneuploidy without sihnificant dirrference.