Analysis on The Polymorphism of Apolipoprotein E in Cerebrospinal Fluid and Sera of Alzheimer's Disease Patients by Gel Electrophoresis Methods

Phenotyping of apolipoprotein E (apoE) was done by using isoelectric focusing (IEF) in 1 6 patients with early-onset Alzheimer's disease(e-AD), 44 patients with late-onset AD (1-AD), 32 patients with cerebrovascular dementia (VD) and 102 healthy subjects as control. The genotype frequency of apOE calculated from the phenotype frequency in control group wasε2 0.083, ε3 0.834 and ε4 O.083. The frequency ofε4 in AD patients was 0.282 in e-AD and 0.193 in 1-AD, which were significantly higher than control. Regarding ε2, said to be a defense factor, no statistical difference was found between the groups. With VD, the frequency of ε4 was 0.125, but not statistically higher than control. The polymorphism of apoE in the cerebrospinal fluid(CSF) and serum was analyzed by two-dimensional gel electrophoresis. With CSF, about twice as many spots were found as compared with serum, This may indicate that apoE in CSF is in a more sialylated state. Serum apoE is separated into three forms of asialo-, monosialo- and disialo-type. The serum asialo-type may not have a sugar chain. But the asialo-type of apoE in CSF is separated into two forms having the same pl but different molecular weights. These results suggest that post-translational modification and metabolism of apoE in the brain are different from those in the liver.

アポリポ蛋白E (apoE)

アルツハイマー病 (AD)

脳脊髄液 (CSF)

二次元電気泳動法 (2-DE)

変異アポリポ蛋白E　(variant apoE)