山口医学

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山口医学 Volume 36 Issue 6
published_at 1987-12

Hyperunstable hemoglobin koriyama found in a Japanese girl with severe hemolytic anemia : Tandem insertion of 5 residues in cluding the proximal histidine in the β chain

重症溶血性貧血の日本人少女に見いだされた超不安定血色素Hb Koriyama : β鎖における近位ヒスチジンを含む5残基の重複挿入
Kawata Reiji
Descriptions
A hyperunstable hemoglobin, Hb Koriyama, was discovered in a 17-years-old Japanese female suffering from asevere chronic hemolytic anemia. The abnormal hemoglobin formed a barely perceptible minor band between Hb A and Hb F in isoelectric forcusing of the hemolysate.The abnormal β chain emerged as a minor peak with very high specific activity between the normal β and δ chain in CM-cellulose olumn chromatography of the ^3H-leucine-labeled globin. It was S-acetamidated and digested with a protease from staphylococcus aureus V_8. Reverse phase high performance liquid chromatography of the digest revealed an abnormal fragment. Amino acid analysis and Edman degradation of the abnormal V_8 peptide established that a segment consisiting of five residues (either Leu-His-Cys-Asp-Lys, His-Cys-Asp-Lys-Leu or Cys-Asp-Lys-Lue-His) was repeated in tendem. The parents and siblings of the proposita were hematologically normal.