Studies on the Quantitative Analysis of Some Poisons by Means of the Thin-Layer Chromatography Part 2. Investigation of Colour Reagents for Detection of Hyponotics and Antipyretic Analgestics etc.

The previous investigation had shown that algebraic determination by the thin-layer chromatography could be applied to some poisons. In the present paper, colour　reagents which were especially suitable to the algebraic method were carried out for determination of hyponotics and antipyretic analgestics etc. The effects of heating and irradiating of urtraviolet rays on the colouration were also studied. Mercurous nitrate, uranine, and dragendorff reagents were remarkably reacted with barbituric and derivatives, bromoureide, and nitrogenheterocyclic compounds, such as antipyrin, 2-methy1-3-o-tolyquinazone, and others, respectively, to form distinct spots. They were therefore recognize as the most suitable colour reagents for determination of the compunds above-mentioned. the colourations by silver nitrate-ammonia, uranine, and Ehrlich's reagents were intensified by heating and that of mcrcurous nitrate, by irradiating of ultraviolet rays.