Memoirs of the Faculty of Engineering, Yamaguchi University

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Memoirs of the Faculty of Engineering, Yamaguchi University Volume 31 Issue 2
published_at 1980

A computer system identifying bacteria by monitoring colony forming process

コロニー形態の時間変化に着目した細菌自動検査の試み
Yamada Takehito
Hashimoto Hajime
Ebina Yoshio
Miyaji Takaoki
fulltext
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KJ00000156363.pdf
Descriptions
A measuring and data processing system is made up by using microcomputer set (PC-8001), TV camera combined with microscope, and digital memory (DM 301) as A/D transducer. The optical source condition is discussed for observing stereo-type microscopical pictures. The colony forming process is examined by the system to get characteristic parameters of bacteria. The examined bacteria are typical nine coliform ones isolated from clinical sample. The culture medium is Brain Heart Infusion ager plate. As characteristic parameters, we propose the time course of colony area and volume which are automatically estimated from the digital pictures. The identification of nine bacteria is tried by using these parameters, and gives good result.