The field of stratified fluid, where the upper layer faces lower one with distinct density interface, exists in the upstream portion of the sluice gate. In order to determine the critical condition of selective withdrawal of lower layer through the sluice gate, experiments and analytical considerations have been taken. Connecting the electrical conductivity probe at the lip of the sluice gate, the density of the withdrawal fluid is measured. In theoretical consideration the model of the density distribution profile at the gate section which changes corresponding to the process of flowing out is introduced, and the density of flowing out fluid is expressed by the parameter ”m”. According to these assumptions, the critical condition of selective withdrawal of lower layer alone could be expressed by the equation (23) or (24). Analytical solution with the model and assumptions must be examined in more detail.