Vehicular speed is an important consideration in road transportation because it is associated with a level of service of road. Speed characteristics of traffic flow are influenced by various aspects of driver, vehicle, road, traffic, regulation and environment. In this paper, we investigated influences of two variables such as number of lanes and regulatory speed on speed distribution of the Yamaguchi By-pass. The data used in this study were obtained by observations carried out corresponding with changes of number of lanes and regulatory speed of the road.