Experimental Study of Cold Blood Cardioplegic Solution. Is Cold Blood Cardioplegic Solution made Effective by the Red Blood Cell?

This sutudy was designed to distinguish whether the efficacy of blood cardioplegic solution is due to a celluler or plasma component. Japanese white rabbits' hearts were preserved for 3 hours by coronary perfusion with oxygenated crystalloid cardioplegic solution (group C), oxygenated blood cardioplegic solution (group B) and oxygenated crystalloid cardiopledic solution with plasma (group P). The coronary perfusion pressure was maintained at cmH2O, and myocardial temperature was maintained at 4℃. The isovolemic left ventricular functions after preservation in group B were better than those in group C. Coronary resistance aftter preservation in group C increased significantly compared to that in group B. O2 consumption, perfusate CPK-MB levels, ultrastructural appearance and water content did not differ significantly amang three groups. The blood cardioplegic solution was better for protecting the myocardium against ischemia. The efficacy might be not due to the oncotic pressure of plasma protein and oxygen supply but to some sther properties of red blood cell, for example, as microparticles which facilitate capillary perfusion.

Cold Blood Cardioplegia

Oncotic Pressure

Olasma

Red Blood Cell