The Production of Hemagglutinin of Jananese B Enceohalitis Virus in Tissue Cultures

Cytopathic effects (CPE) of hamster-Kidney, chick-embryo and porcine-kidney cell cultures (HKTC, CETC, PKTC), hemagglutnatiion tests (HA) on the culture media, 50% Lethal dosis (LD_<50>) with mice, and hemadsorption tests (HAD) on the cultured cells were successively investigated. The results obtained were ad follows: (1) After 72hrs of virus inoculation, CPE were observed on HKTC and PKTC. A significant change was appeared on CETC. (2) The production of hemagglutinin an the result of LD_<50> were nearly correlated. The maximum titers (1:160) of HA were obtained from the media, elapsed from 72 to 96 hrs after virus inoculation, while the values of LD_<50> of the same periods were found at levels 6.4 to 7.5. (3) Before the appearances of hemagglutinin and the values of LD_<50>, hemadsorption were observed in each of cell cultures. The maximum titers of HAD were obtained from the cells, elapsed from 24 to 48 hrs efter virus inoculation. (Received Jun 30, 1975)