Bulletin of the Faculty of Agriculture, Yamaguchi University

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Bulletin of the Faculty of Agriculture, Yamaguchi University Volume 22
published_at 1971

A New Method for the Testing of Germinability of Cereal Crop Seed (Preliminary Resport)

穀物種子の発芽力鑑別に関する新方法(予報)
Utsunomiya Hiroshi
Descriptions
1. A new staining method was studied on the judgement of germinability of hulled rice and whest seeds. 2. It was observed that the embryo and the external parts of the endosperm of live and dead seeds were differently stained with one percent solution of o-methory phenol, one percent solution of neutral red and one percent solution of potassium hydroxide. That is, the embryo of live seeds stained red and the endosperm and embryo of dead seeds stained yellow (Figs. 1,2). It seemed that red staining was induced by the reduction of neutral red by living cells and dead cells remained yellow staining owing to their inability of reductio of neutral red.