In order to obtain the basic data of serum and plasma protein for the veterinary clinical application serum and plasma protein values of thealthy dogs were examined by cellulose acetate membrane electrophoresis. Total number of 372 dogs, including of embryos and newborn puppies, were used. Serum and plasma protein examined every one week for 6 months. Serum protein of dog separated into 7 bands, namely, Albumin, α_1-Globlin, α_2-Gl., β_1-Gl., β_2-Gl., β_3-Gl. And γ-Gl.. The fractionation values of serum and plasma protein were indicated in Table 2~8 and Fig. 2~10. In embryos and newborn puppies before sucking γ-Gl. Was not revealed at all, however, in the puppies which were given colostrum γ-Gl. Value was elevated rapidly after sucking.