On the Seed Globulin of Cucurbitaceae －Report 2. Amino Acid Composition of the Globulin-

1. A comparative study by ion exchange chromatography was made of the amino acid composition of the seed globulin from different species in the family Cucurbitaceae, comprising Cucumis melo, L., Cucumis melo, L.v.,Cucumis melo v.conomon f.albus, Cucumis sativus, Lagenaria vulgaris var.,Luffa cylindrica, Citrullus vulgaris and Cucurbita pepo. 2. Most of the seed globulins soexamined were relatively rich in arginine, aspartic acid and glutamic acid. 3. The amino acid composition of seed globulin was nearly the same for fourdifferent varieties of the genus Cucumis melo and Cucumis sativus. Cucumis, citrullus, Lagenaria and Luffa seemed to differ one from the other. 4. The total nitrogen contents of their seed globlin were ranged from 17.94 to 18.61 per cent. In the nitrogen content of their seed globulins, the varieties of Cucumis melo were the same with one another and Cucumis sativus variety differed from Cumcumis melo. 5. In all of them glycine, alanine and glutamine were found as N-terminal amino acid and glycine, alanine and glutamine as C-terminal acid, thus no considerable difference in the terminal amino acid was recognizable.