DISTRIBUTION OF ALKALINE PHOSPHATASE IN THE PROTEINS OF BOVINE LYMPH-NODES

Homogenate of bovine lymph-nodes was extracted with the same volime of physiological saline solution and acidified to pH 4.5. The extract obtained was fractionated with ethanol. The ethanol fraction obtained by the addition of one third to one half of its volume of alcohol was extracted with water and fractionated by electrophorsis convection, using citrate buffer of pH 6.0 and ionic strenght 0.05. Thephosphatase-activity per unit of nitrogen was higher for the fraction from the top reservoir than for the one from the bottom reservoir. All the fractions obtained at different stages in the procedure of purification were analyzed by electrophoresis. It was found that the alkaline phosphatase of bovine lymph-node was contained in the protein fraction of lower mobility.