A Simple and Rapid Procedure for the Detection of Glutamate-Oxaloacetate Transaminase (GOT) Isozymes of Human Tissues by Agar Gel Eleectrophoresis

By combining the agar gel electrophoresis method with the method of specifically demonstrating oxaloacetate using diazonium salt, a simple method was developed for the detection on glutamate-oxaloacetate transaminase (GOT) isozyme of human organs. This method showed two types of GOT isozyme to be present in human organs. In the heart, liver, kidney, spleen, stomach and pancreas there were the band between α_2 globulin fraction and β-globulin fraction and γ-globulin fraction. In skeletal muscle only one band in the position corresponding to γ-globulin fraction was noted. In the small intestine and lung there was also one band in the position intermediate between the position corresponding α_2globulin and β-globulin fraction of the serum. Acknowledgement: The author wishes to thank professor S. Shibata and associate professor H. Takahashi for constant interest and guidance in this study.