Agglutination of Ax Blood Cells with So-called Anti-C agglutinin in Human O sera

From group B blood cells agglutinated by rabbit anti-human A blood cell sera, eluates of immune anti-C agglutinin were prepared. This fluids agglutinated A and B blood cells to almost the same extent but not agglutinate group O blood cells. Namely, Ax blood cells have C antigen in common with group A and B blood cells. Agglutinins (naturally occurring antibody) in group O human sera that agglutinate Ax blood cells were neutralized by group A, B and AB human salivas which had C antigen in common but not by group O human salivas which were without the common antigen. In other words, the agglutination of Ax blood cells takes place by interaction between C antigen and anti-C agglutinin. Group O human sera agglutinate Ax blood cells with almost no exception. The soundness of Wiener's C-theory was thus demonstrated.